Glucocorticoids (GCs) are widely used immunosuppressive drugs for autoimmune diseases, although considerable gaps exist between current knowledge of the mechanisms of GCs and their conclusive immune-regulatory effects.Here we generated a single-cell transcriptional immune cell atlas based on prednisone-treated or untreated experimental autoimmune uveitis (EAU) mice.Immune cells were globally activated in EAU, and prednisone partially reversed this effect in L-LYSINE 500MG terms of cell composition, gene expression, transcription factor regulation, and cell-cell communication.Prednisone exerted considerable rescue effects on T and B cells and increased the proportion of neutrophils.
Besides commonly regulated transcriptional factors Riding Coats (Fosb, Jun, Jund), several genes were only regulated in certain cell types (e.g.Cxcr4 and Bhlhe40 in T cells), suggesting cell-type-dependent immunosuppressive properties of GC.These findings provide new insights into the mechanisms behind the properties and cell-specific effects of GCs and can potentially benefit immunoregulatory therapy development.